A trimodal atomic force mircoscope probe sensor for the physiological investigation of pores in native cell membranes

Colloquial Talk of Dr. Sarmiza Stanca
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Physics Colloquium
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Abbeanum
Fröbelstieg 1, Hörsaal 1
07743 Jena
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Physikalisch-Astronomische Fakultät
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Dr. Sarmiza Stanca

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Dr. Sarmiza Stanca

A trimodal atomic force mircoscope probe sensor for the physiological investigation of pores in native cell membranes

The work aims to develop a trimodal atomic force microscopy (AFM) probe sensor, which synergistically combines the nanoscale topography recordings with nanoscale tip-electrochemistry recordings, to obtain nanoscale electro-structural information, and with photoinduced force microscopy (PiFM) recordings, to obtain vibrational modes of the molecules. The set-up conducted under the illumination of two lasers beams, complementarily combines tree sensitive techniques: I. Atomic force microscopy for morphological information at nanoscale, II. Photoinduced force microscopy for molecular vibrational information at nanoscale, and III. Tip electrochemistry for electrochemical information at nanoscale. This work benefits from photoinduced force microscopy, which offers great potential for investigating quantum phenomena at the nanoscale between tip and membrane.

Schematic representation of a proposed cell setup combining three sensitive techniques: I. atomic force microscopy, II. photoinduced force microscopy (PiFM), and III. tip electrochemistry (WE– working electrode, CE – counter electrode, A – ampere meter, V – voltmeter, Δz – tip deflection, ΔU– voltage bias, ΔI – current intensity difference). One laser is used for feedback imaging. The light from the second laser irradiates the tip and the cell membrane, inducing a photomechanical oscillation that is detected by the AFM tip and consequently by the quadrant photodiode.

Picture: Dr. Sarmiza Stanca